Study on EcoRI And HinDIII Immobilization Using Sodium Alginate and Their Restriction Activity on Lambda DNA

Biotechnology, often known as genetic engineering or recombinant DNA technology, is the process of creating artificial DNA by inserting one or more DNA strands. By altering microorganisms and animals to create medicinally valuable substance(s), it opens up new possibilities for breakthroughs in the production of diverse therapeutic goods in the fields of genetics and biomedicine. The location of the chosen gene's insertion into the vector genome is largely determined by the restriction enzyme employed in rDNA technology. Depending on the cost, the industrial use of enzymes is restricted since more expensive enzymes are not cost-effective due to their poor reusability factor. To get around these restrictions, an effort was made in this study to immobilize the enzymes, specifically EcoRI and HinDIII, by employing the entrapment approach with sodium alginate as a matrix. The effectiveness of the immobilized enzymes' action on Lambda DNA and their reusability were assessed. Our findings clearly imply that the chosen immobilized enzymes have good activity and are reusable.