Green Synthesis and Comparative Antimicrobial Evaluation of The Extract of Ruta Graveolens

In the present study the aqueous extract of the leaves of Ruta graveolens plant was investigated for its phytochemical constituents. The extract was used to synthesize silver nanoparticles and the comparative antibacterial efficacy of the extract as well as silver nanoparticles were evaluated against two micro-organisms using agar diffusion method. Microorganisms selected were Acinetobactor baumannii and Enterococcus. Results showed that the aqueous extract of the leaves contained alkaloids, carbohydrates, tannins, flavonoids, reducing sugar, proteins and phytosterols. Comparative evaluation of the antibacterial efficacy revealed that the synthesized silver nanoparticles exhibited more efficacy with maximum zone of inhibition ranging from 20-35mm were as the zones of inhibition ranging of the extract was found to be in the range of 2-20mm.