Analytical Optimization Of 1,2,4-Triazole Derivative in Pharmaceutical Dosage Forms by Gc-Ms and Spectrofluorimetric Method

This article mainly emphasizes on simple, effective and selective analytical procedures especially two methods adopted for quantification of Letrozole in pharmaceutical dosage forms by using gas chromatography with mass spectrometry and spectrofluorimetric techniques. Method 1: GC-MS involves separation using column with temperature programming. The EI mass spectrum was characterized by [M]+ at 416 and a base peak at m/z 73 for Letrazole respectively. Quantification of the analytes were based upon estimating peak areas. Method 2: The suggested method is depended upon measuring the relative fluorescence intensity in distilled water and methanol (40%v/v:60%v/v) at an excitation and emission wavelength of 364nm and 407nm respectively. The reliability and analytical performance of the mentioned methods includes linearity, ranges, precision, accuracy, robustness, ruggedness and quantification limits were validated statistically. The proposed methods were successfully applied for the determination of Letrazole in commercially available tablet formulations.