Antibody Characterization Report for SLC22A14

This article presents antibody characterization data that can be utilized as a guide to select high-performing antibodies for Solute carrier family 22 member 14 (SLC22A14), encoded by the SLC22A14 gene. Six commercial human or mouse reactive antibodies were evaluated by western blot following a standardized protocol which compares read-outs in isogenic parental controls and knockout cell lines. The antibodies were tested by western blot on WT and SLC22A14 KO from mice testis to recognize mouse SLC22A14 as well as a SLC22A14 overexpression line from RESOLUTE consortium to recognize human SLC22A14. Thank you to Dr. Keiichiro from Yogo Shizuoka University, Japan for donating the mouse testis lysates. We'd also like to thank the Bill and Melinda Gates Foundation for their support and nomination of this target as well as the Structural Genomics Consortium for coordinating this study.

Institutions

Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2024-08-30
DOI
https://doi.org/10.5281/zenodo.13618715
Primary Topic
Sperm and Testicular Function
Type
article
Field-Weighted Citation Impact
0.00
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article

Antibody Characterization Report for SLC22A14

Zenodo (CERN European Organization for Nuclear Research)
Sperm and Testicular Function
article

Antibody Characterization Report for SLC22A14

article en

Abstract

This article presents antibody characterization data that can be utilized as a guide to select high-performing antibodies for Solute carrier family 22 member 14 (SLC22A14), encoded by the SLC22A14 gene. Six commercial human or mouse reactive antibodies were evaluated by western blot following a standardized protocol which compares read-outs in isogenic parental controls and knockout cell lines. The antibodies were tested by western blot on WT and SLC22A14 KO from mice testis to recognize mouse SLC22A14 as well as a SLC22A14 overexpression line from RESOLUTE consortium to recognize human SLC22A14. Thank you to Dr. Keiichiro from Yogo Shizuoka University, Japan for donating the mouse testis lysates. We'd also like to thank the Bill and Melinda Gates Foundation for their support and nomination of this target as well as the Structural Genomics Consortium for coordinating this study.

Zenodo (CERN European Organization for Nuclear Research)
Montreal Neurological Institute and Hospital (CA), McGill University (CA)
Openalex Percentile: Top 57%
Sperm and Testicular Function
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Antibody Characterization Report for SLC22A14 · Zenodo (CERN European Organization for Nuclear Research) (2024) | TGRS Research Map | TGRS