Nigrograna magnoliae Wanas

Nigrograna magnoliae Wanas, PLoS One, 15 (7): 10 (2020) Fig. 2 Description. Saprobic on dead branches of Buxus sinica (Buxaceae). Sexual morph: Ascomata 204–326 μm wide, 140–220 μm high (x ̅ = 248 × 187 μm, n = 20), solitary or gregarious, scattered, immersed to semi-immersed, with only ostiolar necks visible on the host surface, trigonoid, uniloculate, perithecioid, globose to subglobose, brown to dark brown, with an ostiole. Ostiole central or eccentric, brittle. Peridium 15–23 μm (x ̅ = 18 μm, n = 20) composed of angular cells, consisting 4–5 layers, brown to dark brown thick-walled cells of outer layer, hyaline to subhyaline thin-walled cells of inner layer. Hamathecium 1–3 μm (x ̅ = 2 μm, n = 20) wide, composed of numerous, filamentous, hyaline, aseptate or separate, rarely branched, smooth-walled pseudoparaphyses. Asci 57–103 × 8–11 μm (x ̅ = 72.5 × 10 μm, n = 30), 8 - spored, bitunicate, fissitunicate, clavate to long cylindric-clavate, short cylindrical pedicellate with a swollen base, apically rounded, with a minute ocular chamber. Ascospores 13–19 × 4.5–6 μm (x ̅ = 14.5 × 5 μm, n = 50), 1–2 - seriate, partially overlapping, fusoid to ellipsoid, tapering towards the blunt ends, or blunt at both ends, guttulate, smooth-walled, olivaceous to yellowish-brown when young, 1 - septate; deeply constricted at septa, becoming 3 - septate, brown to dark brown when mature, without appendages. Asexual morph: Undetermined. Culture characteristics. Ascospores germinated on PDA within 24 h, and germ tubes produced from basal cell. Colonies growing on PDA reached 32-33 mm in diameter after three weeks at 25 ° C in dark, white in the whole colony from above, and slightly raised in the center, circular, flat, edge entire, margin well-defined; in reverse, grayish black in the center, off-white at the margin, the color gradually lightens from center to edge, no pigmentation on PDA. Material examined. China • Yunnan Province, Kunming City, Panlong District, Kunming Botanical Garden. 25°8'27′′N, 102°44'24′′E, elevation 1,922 m, on dead branches of medicinal plant Buxus sinica (Rehder & E. H. Wilson) M. Cheng (Buxaceae), 11 November 2022, H. Z. Du, S 735 (HUEST 23.0203), living culture UESTCC 23.0203; • ibid., Sichuan Province, Chengdu City, High-tech West District, Yaobo Park, 30°43'57′′N, 103°56'21′′E, elevation 504 m, on dead branches of medicinal plant Eucommia ulmoides Oliv. (Eucommiaceae), 11 August 2021, H. Z. Du, S 347 (HUEST 23.0206), living culture UESTCC 23.0206; • ibid., Guizhou Province, Guiyang City, Nanming District, Guiyang Medicinal Botanical Garden, 26°32'18′′N, 106°41'48′′E, elevation 1,127 m, on dead branches of medicinal plant Mahonia bealei (Fort.) Carr. (Berberidaceae), 12 October 2021, H. Z. Du, S 370 (HUEST 23.0207), living culture CGMCC 3.25627 = UESTCC 23.0207; • ibid., Guizhou Province, Guiyang City, Huaxi District, 26°30'43"N, 106°39'32"E, elevation 1,155 m, on dead branches of medicinal plant Camellia sinensis (L.) O. Ktze. (Theaceae), 2 February 2023, Y. X. Yu, GY 33 (HUEST 23.0190), living culture UESTCC 23.0190. Notes. Nigrograna magnoliae was introduced by Wanasinghe et al. (2020) with both asexual and sexual morphs reported in China. The host distribution of this species is presented in Table 2. Our collections are identical to N. magnoliae based on morphology and phylogeny. Therefore, we reported it as new host records from medicinal plants of Buxus sinica, Camellia sinensis, Eucommia ulmoides and Mahonia bealei in China.