A Comparative Analysis of Embryo Quality and Clinical Pregnancy Following Culture of Embryos by Conventional Culture System and Uninterrupted Culture System

Objective: The current study was done to compare the embryo quality of an uninterrupted embryo culture compared to conventional embryo culture and assessment on clinical outcomes in couples undergoing Assisted Reproductive Technology (ART). The study will compare the fertilization and cleavage rates and implantation rate between conventional culture system and uninterrupted culture. Methods: A total of 1000 patients were included in this study who have enrolled for ART for various etiologies, among which 500 patients (Group A) belonged to conventional culture system and 500 (Group B) belonged to the uninterrupted culture system group who fulfilled the criteria were included in the study. Embryo morphology evaluation in group-A. After ICSI, injected oocytes are transferred to 4- well dish kept in groups with 1-well filled with 500μl of cleav media kept in standard bench top incubator (MINC). Results: The study found no significant age difference between Group A (mean age 32 years) and Group B (mean age 31 years). Male factor infertility was more common in Group A, while female factor infertility was more common in Group B, though neither difference was significant. Group B showed better outcomes in embryo quality, implantation, and ongoing pregnancy rates, despite Group A having better oocyte and sperm grading. Fertilization and cleavage rates were similar, but Group B had more top-quality embryos and a significantly higher number of embryos per patient, indicating the benefits of an uninterrupted culture system. Conclusion: This study shows that uninterrupted culture systems can enhance embryo quality, implantation efficiency and clinical pregnancy rate in ART. Consequently, these findings pose the uninterrupted culture system as a plausible candidate for enhancing ART success, which may translate into improved overall patient outcomes in fertility treatments.