Anti- Inflammatory Insights: MTT Assay of Vedi Annabedhi Cgendooram on LPS-Induced ELT-3 Cell Lines

Siddha medicine, a traditional healing practice rooted in Tamil culture, recognizes and categorizes over 4,448 diseases. Among its formulations, Vedi Annabedhi Chendhooram (VABC), a herbo-mineral medicine, is notable for its potent effects on ailments such as jaundice, anemia, dysentery, fever, dropsy, ascites, and generalized anasarca. Traditional Siddha practitioners also use VABC for uterine disorders, including dysmenorrhea and leiomyomas (uterine fibroids,) where inflammation plays a crucial role in disease progression. This study investigates the anti-inflammatory effects of VABC on uterine leiomyomas. Methods: An in vitro study was conducted using lipopolysaccharide (LPS)-induced Eker Leiomyoma Tumor-3 (ELT-3) cells to assess the anti-inflammatory effects of Vedi Annabedhi Chendhooram (VABC).