Molecular genetic analysis of gene pools of rare and endangered plant species Adonis L. of Northern Kazakhstan

Adonis L. is one of the largest and most famous genera of the Runanculaceae family. Adonis genus includes two parts of annual and perennial species. The latter contains more effective substances than annual species, but among annual species, plants. Adonis L. contains more effective substances, which mainly grow in sandy areas with high heat in the central parts of Kazakhstan. The extract of Adonis L. plant species has a high total phenolic content and, therefore, requires research on this plant's antioxidant and radical removal properties. Describing plants by molecular markers is a good strategy for improving and protecting plant genetic resources. The aim of this study is molecular genetic analysis to investigate the total phenolic content of the Adonis L. hydroalcoholic extract. The phenolic content of this plant extract was determined by the Folin Ciocaltio reagent method (FC) and Gallic acid as a standard. The results showed that the hydroalcoholic extract has a total phenolic content of 26.607 ± 2.35 mg of Gallic acid per gram of extract. The increase in the antioxidant activity of essential oils is significantly related to the increase in the phenolic content of essential oils. The results showed that the presented method is suitable and useful for estimating genetic diversity in Adonis L. populations.