紫外-LiCl联合ARTP诱变选育高产花生四烯酸的 高山被孢霉突变株Breeding of Mortierella alpina mutants with high yield of arachidonic acid by UV-LiCl combined with ARTP mutagenesis

为得到遗传性状稳定、高产花生四烯酸（ARA）的高山被孢霉菌株，以实验室保藏的高山被孢霉FD-1为出发菌株，采用紫外-LiCl联合常压室温等离子体（ARTP）技术进行诱变处理，并进行TTC平板初筛和摇瓶发酵复筛。结果表明，经紫外-LiCl联合ARTP诱变处理，获得一株高产ARA的高山被孢霉突变株Z6-A23。菌株Z6-A23所产油脂中ARA含量高达44.57%，ARA产量达3.62 g/L，分别较出发菌株FD-1提高了75.40%和223.21%。经10次传代培养，突变株Z6-A23代谢合成ARA性能稳定，说明其具有良好的遗传稳定性。综上，突变株Z6-A23有望成为高产ARA的潜力菌株。In order to obtain a genetically stable and high yield arachidonic acid (ARA)-producing Mortierella alpina strain, mutagenesis of the strain of M. alpina FD-1 preserved in laboratory was performed by UV-LiCl combined with atmospheric and room temperature plasma (ARTP) method, and the strain was screened by TTC plate and the fermentation in shaking flasks. The results showed that a mutant strain Z6-A23 with high yield of ARA was obtained by UV-LiCl combined with ARTP mutagenesis. The ARA content in the oil produced by the mutant strain Z6-A23 reached as high as 44.57%, and the ARA yield was 3.62 g/L, which were 75.40% and 223.21% higher than those of the original strain FD-1, respectively. After 10 subcultures, the mutant Z6-A23 had stable ability to synthesize ARA, indicating that it had good genetic stability. In conculsion, the mutant strain Z6-A23 obtained is expected to be a promising strain for efficient ARA production.