Flow chart of cryo-EM image processing and 3D reconstruction for the complex of JN.1 spike ectodomain and Nanosota-9.

<p>Representative raw cryo-EM image and 2D classes are presented. 3D refinements using all the particles from good 3D classes generated a 2.99 Å map. Further local refinement improved the density for the bound nanobody. The angular distribution plot, final maps, half-map FSC curves and accompanying local resolution illustrations are enclosed in the dashed black boxes.</p> <p>(PDF)</p>

Publication Details

Journal
Figshare
Published
2024-11-26
DOI
https://doi.org/10.1371/journal.ppat.1012726.s003
Primary Topic
Advanced Electron Microscopy Techniques and Applications
Type
article
Field-Weighted Citation Impact
0.00
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article

Flow chart of cryo-EM image processing and 3D reconstruction for the complex of JN.1 spike ectodomain and Nanosota-9.

Figshare
Advanced Electron Microscopy Techniques and Applications
article

Flow chart of cryo-EM image processing and 3D reconstruction for the complex of JN.1 spike ectodomain and Nanosota-9.

article en

Abstract

<p>Representative raw cryo-EM image and 2D classes are presented. 3D refinements using all the particles from good 3D classes generated a 2.99 Å map. Further local refinement improved the density for the bound nanobody. The angular distribution plot, final maps, half-map FSC curves and accompanying local resolution illustrations are enclosed in the dashed black boxes.</p> <p>(PDF)</p>

Figshare
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Flow chart of cryo-EM image processing and 3D reconstruction for the complex of JN.1 spike ectodomain and Nanosota-9. · Figshare (2024) | TGRS Research Map | TGRS