S6 Fig -

<p>(<b>A</b>) Protease activity, (<b>B</b>) elastase activity, and (<b>C</b>) PQS/HHQ levels, normalized to cell density (OD<sub>600</sub>), in the supernatants of <i>P</i>. <i>aeruginosa</i> PAO1 and the <i>apaH</i> mutant, carrying the empty plasmid pME6032 or the plasmid pME<i>apaH</i>, cultured at 37°C in LB supplemented with 100 μM IPTG. (<b>D</b>) Growth curves and (<b>E</b>) pyoverdine levels, normalized to cell density (OD<sub>600</sub>), of the same strains cultured at 37°C in the iron-poor medium CAA, supplemented with 100 μM IPTG and 50 μM FeCl<sub>3</sub> when indicated (+ Fe). Values are the mean (± standard deviation) of three independent assays. Asterisks indicate a statistically significant difference (<i>P</i> < 0.001) with respect to PAO1 pME6032 (ANOVA).</p> <p>(PDF)</p>

Publication Details

Journal
Figshare
Published
2024-08-19
DOI
https://doi.org/10.1371/journal.ppat.1012486.s010
Primary Topic
Ocean Waves and Remote Sensing
Type
article
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Abstract

<p>(<b>A</b>) Protease activity, (<b>B</b>) elastase activity, and (<b>C</b>) PQS/HHQ levels, normalized to cell density (OD<sub>600</sub>), in the supernatants of <i>P</i>. <i>aeruginosa</i> PAO1 and the <i>apaH</i> mutant, carrying the empty plasmid pME6032 or the plasmid pME<i>apaH</i>, cultured at 37°C in LB supplemented with 100 μM IPTG. (<b>D</b>) Growth curves and (<b>E</b>) pyoverdine levels, normalized to cell density (OD<sub>600</sub>), of the same strains cultured at 37°C in the iron-poor medium CAA, supplemented with 100 μM IPTG and 50 μM FeCl<sub>3</sub> when indicated (+ Fe). Values are the mean (± standard deviation) of three independent assays. Asterisks indicate a statistically significant difference (<i>P</i> < 0.001) with respect to PAO1 pME6032 (ANOVA).</p> <p>(PDF)</p>

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