<i>kpACE</i> overexpression reduces virulence in a pneumonia model using both female and male mice.

<p><b>(A-D)</b><i>kpACE</i> overexpression (pkpACE) reduces virulence in a pneumonia model using female mice. (A) Survival rates of mice. Bacterial burdens in lungs (B), livers (C), and spleens (D) were indicated, respectively. <b>(E-H)</b> <i>kpACE</i> overexpression (pkpACE) reduces virulence in a pneumonia model using male mice. (E) Mouse survival rates of mice. Bacterial burdens in lungs (F), livers (G), and spleens (H) were indicated respectively. Mouse survival rates were monitored 7 days post intranasal infection with 2,000 CFU of ATCC43816 variants. Bacterial burdens in organs were determined 48 h post intranasal inoculation. Each dot represents one mouse, and dotted lines represent the detection limit. The Gehan-Breslow-Wilcoxon test was performed to compare the survival rates. One-way ANOVA with Dunnett’s multiple comparisons test was performed to determine the statistical significance among bacterial burdens in organs.</p> <p>(PDF)</p>