Influence of RNase III and PNPase on the copy number of the virulence plasmid pYV.

<p><i>Y</i>. <i>pseudotuberculosis</i> strains YPIII (wt), YP139 (Δ<i>pnp</i>), and YP356 (Δ<i>rnc</i>) were grown at 25°C, and 37°C, and the wildtype YPIII also at 37°C/-Ca<sup>2+</sup>, i.e. T3SS/Yop-inducing conditions. The total DNA of the strains grown under the different conditions was prepared and the copy number of the virulence plasmid was determined by qPCR. The relative plasmid copy number of wildtype was compared with the different mutant strains. Data represent the mean ± SD from three independent biological replicates. Significant differences were determined using the Student’s t-test and are indicated by asterisks (* P<0.05).</p> <p>(PDF)</p>