Gating scheme and DENV-1 RVP infection of tmIgM and tmIgA transfected 293T cells.

<p><b>A)</b> Expression and DENV E protein binding activity of VDB33 tmIgM and tmIgA expression constructs. <b>B)</b> Expression and gating of tmIgM and tmIgA expression in transiently-transfected 293T cells <b>C)</b> Representative flow cytometry plots showing the frequency of DENV-1 RVP infected cells within the DC-SIGN, CR261 tmIgG, VDB33 tmIgA, VDB33 tmIgG, and VDB33 tmIgG positive 293T cells 24 hours after infection <b>D)</b> Quantification of DENV-1 RVP infected cells within the DC-SIGN, CR261 tmIgG, VDB33 tmIgA, VDB33 tmIgG, and VDB33 tmIgG positive 293T cells 24 hours after infection</p> <p>(PDF)</p>

Publication Details

Journal
Figshare
Published
2024-10-31
DOI
https://doi.org/10.1371/journal.ppat.1012683.s002
Primary Topic
interferon and immune responses
Type
article
Field-Weighted Citation Impact
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article

Gating scheme and DENV-1 RVP infection of tmIgM and tmIgA transfected 293T cells.

Figshare
interferon and immune responses
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Gating scheme and DENV-1 RVP infection of tmIgM and tmIgA transfected 293T cells.

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Abstract

<p><b>A)</b> Expression and DENV E protein binding activity of VDB33 tmIgM and tmIgA expression constructs. <b>B)</b> Expression and gating of tmIgM and tmIgA expression in transiently-transfected 293T cells <b>C)</b> Representative flow cytometry plots showing the frequency of DENV-1 RVP infected cells within the DC-SIGN, CR261 tmIgG, VDB33 tmIgA, VDB33 tmIgG, and VDB33 tmIgG positive 293T cells 24 hours after infection <b>D)</b> Quantification of DENV-1 RVP infected cells within the DC-SIGN, CR261 tmIgG, VDB33 tmIgA, VDB33 tmIgG, and VDB33 tmIgG positive 293T cells 24 hours after infection</p> <p>(PDF)</p>

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interferon and immune responses
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Gating scheme and DENV-1 RVP infection of tmIgM and tmIgA transfected 293T cells. · Figshare (2024) | TGRS Research Map | TGRS