Top-ranked genes by MAGeCK have low representation in the T<sub>0</sub> pool of cells.

<p>The 3D plot highlights in dark blue the genes that TRACS determined to have low Library ES. The vertical axis represents Library ES. The volcano plots illustrate genes that were found to be essential by MAGeCK-RRA or MAGeCK-MLE (LFC < 0 and unadjusted <i>p</i> value < 0.05; no FDR cutoffs). Dark blue data points in volcano plots indicate genes that TRACS found to have low Library ES, demonstrating that removing the FDR cutoff selects for genes with poor sgRNA representation. In all three plots, genes in red have Library ES > 985 and genes in dark blue have Library ES < 985.</p> <p>(PDF)</p>