Optimisation and efficacy of anti-<i>Mfn1</i> siRNAs on adipogenic differentiation of 3T3-L1s.

<p>Undifferentiated 3T3-L1 pre-adipocytes were treated with three separate Invitrogen Silencer Select siRNAs (CatIDs: s85003 = ‘siRNA03’, s85002 = ‘siRNA02’, s85004 = ‘siRNA01’) for 24-hours, each siRNA was used at 10nmol/L, 30nmol/L, and 50nmol/L. A, Western blot demonstrating Mfn1 protein knock-down efficacy. Following this, 3T3-L1 adipocytes were treated with s85002 (= ‘siRNA02’) and s85004 (= ‘siRNA01’) until day +10 differentiation. B, Relative mRNA expression of markers of adipogenesis at day +10 differentiation. C, Lipid accumulation at day + 10 as measured by fluorescence using AdipoRed. D, Representative images for adipogenic differentiation using brightfield microscopy and AdipoRed staining. Data from n = 2 biological repeats.</p> <p>(PDF)</p>