Acquired phosphorus deficiency by low dietary phosphorus intake.

<p>Eight-week old C57BL/6J male mice were fed a diet containing 0.02% inorganic phosphorus (LP) for 2 weeks and compared to age matched C57BL/6J male mice fed normal phosphorus diet (0.6% Pi; CONT). Serum and tissue samples were collected at the end of the experiment. <b>(A)</b> Serum phosphorus levels, <b>(B)</b> Quantitative real-time RT-PCR for renal <i>Cyp27b1</i> expression. Data are expressed as fold change (2-ΔΔCt) relative to housekeeping gene <i>Hprt</i>. <b>(C)</b> Serum calcium levels, and <b>(D)</b> Circulating PTH levels measured by ELISA. All data were analyzed for normality with Shapiro-Wilk test, homogeneity of variance by F test, and unpaired t test was performed. For samples with normal distribution and equal variances, unpaired t test was performed compared to WT (A). When the samples were in normal distribution but not in homogeneity of variance, the data were analyzed by Welch’s t test (B, C, D). *P < 0.05, **P < 0.01, ***P <0.001 compared to CONT (control diet).</p> <p>(PDF)</p>