Normalized eDNA reads from high-throughput sequencing of samples.

Environmental DNA index (normalized using the inversed order of operations of the Wisconsin double-standardization) from molecular operational taxonomic units (MOTUs) that were amplified and sequenced using the assay. Amplicons were sequenced on an Illumina MiSeq platform and matched with NCBI’s BLASTn algorithm (%ID > 97, E-value < 1e-10). <i>Chondria tumulosa</i> matched 17 MOTUs and six MOTUs matched to the family Rhodomelaceae, tribe Polysiphonieae. Bar colors refer to the categorization of <i>C</i>. <i>tumulosa</i> from visually surveyed sites (green: “Absent”, orange: “Present”). Samples were from Kuaihelani (“K”, or Midway Island), Manawai (“M”, or Pearl & Hermes Atoll), or Oʻahu (“OA”). (DOCX)