<i>Tg</i>ATM1 and <i>Pf</i>ATM1 dimerization.

<p>(<b>A</b>-<b>C</b>) Controls for Co-immunoprecipitation assay shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012593#ppat.1012593.g005" target="_blank">Fig 5A-5C</a>. Western blots as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012593#ppat.1012593.g005" target="_blank">Fig 5A-5C</a> but membranes were probed with anti-myc antibody. <i>Tg</i>ATM1-myc (A), <i>Pf</i>ATM1-myc (B) and <i>Sc</i>ATM1-myc (C) are indicated. Blots are representative of three independent biological replicates. (<b>D</b>) Recombinant <i>Pf</i>ATM1-CTD dimer breaks down into monomers upon treatment with DTT and urea. (<b>E</b>) Size exclusion chromatography on S200 column for purification of <i>Pf</i>ATM1 separates dimeric (P1) and monomeric protein with some degradation (P2). Absorbance at 280 nm is plotted against elution volume. Coomassie-stained SDS-PA gel for P1 and P2 is shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012593#ppat.1012593.g005" target="_blank">Fig 5F</a>. Inset, molecular weight standard plot for S200. (<b>F</b>) CD spectra of purified <i>Pf</i>ATM1 homodimer.</p> <p>(PDF)</p>