RNA binding of ORF6 <i>in vitro</i>.

<p><sup>32</sup>P-labeled U1 snRNA was incubated with T7-ORF6-His (1 μg) at 30°C for 20 min. After the incubation, the sample was irradiated with UV light (200 mJ/cm<sup>2</sup>), and treated with RNase A. The sample was immunoprecipitated using an antibody against the T7 tag or the Myc tag. The immunoprecipitated protein was analyzed by SDS-PAGE and autoradiography.</p> <p>(PDF)</p>

Publication Details

Journal
Figshare
Published
2024-10-31
DOI
https://doi.org/10.1371/journal.pone.0312098.s004
Primary Topic
RNA regulation and disease
Type
article
Field-Weighted Citation Impact
0.00
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article

RNA binding of ORF6 in vitro.

Figshare
RNA regulation and disease
article

RNA binding of ORF6 in vitro.

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Abstract

<p><sup>32</sup>P-labeled U1 snRNA was incubated with T7-ORF6-His (1 μg) at 30°C for 20 min. After the incubation, the sample was irradiated with UV light (200 mJ/cm<sup>2</sup>), and treated with RNase A. The sample was immunoprecipitated using an antibody against the T7 tag or the Myc tag. The immunoprecipitated protein was analyzed by SDS-PAGE and autoradiography.</p> <p>(PDF)</p>

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RNA regulation and disease
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RNA binding of ORF6 <i>in vitro</i>. · Figshare (2024) | TGRS Research Map | TGRS