Validation of RNAseq data by RT-qPCR.

<p>a. RNAseq expression data from 11 rice genes in response to JA treatment in WT, oscoi1ab and oscoi2 plants. Asterisks indicate Differentially Expressed Genes as determined by EdgeR (log2FC ≥1 or ≤-1 and FDR ≤0.01). b. RT-qPCR values obtained from the same genes. Bars represent the mean of log2(relativeFC) ± SE from five biological replicates. Asterisks indicate significant differences between control and JA treated plants (n = 5, t-test, p-value ≤0.01).</p> <p>(DOCX)</p>

Publication Details

Journal
Figshare
Published
2024-10-28
DOI
https://doi.org/10.1371/journal.pone.0311136.s003
Primary Topic
Plant responses to water stress
Type
article
Field-Weighted Citation Impact
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article

Validation of RNAseq data by RT-qPCR.

Figshare
Plant responses to water stress
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Validation of RNAseq data by RT-qPCR.

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Abstract

<p>a. RNAseq expression data from 11 rice genes in response to JA treatment in WT, oscoi1ab and oscoi2 plants. Asterisks indicate Differentially Expressed Genes as determined by EdgeR (log2FC ≥1 or ≤-1 and FDR ≤0.01). b. RT-qPCR values obtained from the same genes. Bars represent the mean of log2(relativeFC) ± SE from five biological replicates. Asterisks indicate significant differences between control and JA treated plants (n = 5, t-test, p-value ≤0.01).</p> <p>(DOCX)</p>

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Plant responses to water stress
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