Additional evolved strains with LOH at <i>KSR1</i>.

<p>(A) Read depth from whole genome sequencing normalized to average depth across the whole genome for three evolved strains that all contain LOH at the <i>KSR1</i> locus and their SC5314 progenitor. Gray bars show heterozygous positions. (B) Microscopy of progenitor and evolved strains grown at 37°C for 2 hours shows differences in the initiation of hyphal growth. (C) Survival curves for <i>G</i>. <i>mellonella</i> show a reduction in virulence for all three evolved strains relative to the progenitor (Log-rank test, ** <i>P</i> < 0.01). (D) Median <i>G</i>. <i>mellonella</i> survival over 14 days demonstrates that treatment with FLC increases survival of the wild-type strain (gray) but does not increase survival for larvae infected with evolved strains LOH3 (green) and LOH4 (blue).</p> <p>(PDF)</p>