MBD deletion abrogates the ability of MeCP2 to recruit LANA.

(A) SLK cells were transfected with dCas9-SunTag, scFv-MeCP2, GFP-LANA, and sgTelomere. Different scFv-MeCP2 constructs were transfected to express wild-type (wt), T158M mutant, or a MBD deletion mutant of MeCP2, as illustrated on the left. An immunofluorescence assay detected scFv-MeCP2, or fluorescently labelled GFP-LANA. The nucleus was stained with DAPI. Scale bar = 5 μm. The plots of the red, green, and blue pixel intensities along the white arrow (in the middle panels) are presented. Images are representatives of at least three independent experiments. (<b>B</b>) Pearson’s correlation coefficient was determined for 15 cells in each treatment and presented as box and whiskers (min to max). Two-tailed <i>t</i> tests were performed (*, P ≤ 0.05; **, P ≤ 0.01; ***; P ≤ 0.001, ****; P ≤ 0.0001). (PDF)