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<p><b>S1 Fig.</b> Median Chl <i>a</i> concentrations in the different treatments during pre-bloom (A), bloom (B), and post-bloom periods (C). Blue indicates the control treatment (C), red, green, and yellow indicated the heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. Boxplots with different letters differ are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has no stars or letters. <b>S2 Fig.</b> Median nutrient concentrations in the mesocosms between the different treatments for pre-bloom, bloom, and post-bloom periods. Nutrients are NH<sub>4</sub><sup>+</sup> (A, B, and C), NO<sub>2</sub><sup>-</sup> (D, E, and F), NO<sub>3</sub><sup>-</sup> (G, H, and I), SiO<sub>2</sub> (J, K, and L), and PO<sub>4</sub><sup>3-</sup> (M, N and O). Blue, control treatment (C); red, green, and yellow, the heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). A lack of asterisks and letters indicates RM-ANOVAs was nonsignificant. <b>S3 Fig.</b> Median concentrations of taxonomic pigments in the different treatments during pre-bloom, bloom, and post-bloom periods: Chl <i>b</i> (A, B, and C), Prasinoxanthin (D, E, and F), Zeaxanthin (G, H, and I), Alloxanthin (J, K, and L), 19HF (M, N, and O), Fucoxanthin (P, Q, and R), and Peridinin (S, T, and U). Blue, control (C); red, green, and yellow, heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post-hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has no stars or letters. <b>S4 Fig.</b> Median abundance of phytoplanktonic groups in the different treatments during pre-bloom, bloom, and post-bloom periods: Cyano (A, B, and C), Picoφ (D, E, and F), and Nanoφ (G, H, and I). Blue, control (C); red, green, and yellow, heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has neither stars nor letters. <b>S5 Fig.</b> Median abundance of protozooplankton (A, B and C) and micrometazooplankton (D, E and F) in the different treatments during pre-bloom, bloom, and post-bloom periods. Blue, control (C); red, green, and yellow, heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has neither stars nor letters. <b>S6 Fig.</b> Redundancy Analysis (RDA) between phytoplankton composition (pigment and cytometry and environmental parameters. (A) Pre-bloom period, (B) Bloom period, and (C) Post-Bloom periods. Dots present sampling dates community composition. Blue arrows present the projections of environmental parameters. Red labels present projection of the pigments and cytometry groups. The proximity between environmental parameters or pigments and cytometry groups and sampling dates indicates characteristic associations. Ellipses represents 95% confidence distribution interval of community for each treatment (C, T, MicroZ and TMicroZ). Ellipses were not represented for Pre-bloom (A) because they were overlapping and communities showed no significant differences. <b>S1 Table</b>. Light penetration mean in the mesocosms and diffuse attenuation coefficient (Kd). <b>S2 Table</b>. Statistical results for nutrients (NH<sub>4</sub><sup>+</sup>, NO<sub>2</sub><sup>-</sup>, NO<sub>3</sub><sup>-</sup>, Si, PO<sub>4</sub><sup>3-</sup>) concentration differences between treatments on two factors (Temperature x Zooplankton community [Zoo. Comm]), for Pre-Bloom, Bloom and Post-Bloom periods. Bold values indicate significant tests (p-values < 0.05). <b>S3 Table</b>. Statistical results for Pigments (Chl <i>b</i>, Prasi, Zea, Allo, 19HF, Fuco, Peri) concentration differences between treatments on two factors (Temperature x Zooplankton community [Zoo. Comm]), for Pre-Bloom, Bloom and Post-Bloom periods. Bold values indicate significant tests (p-values < 0.05). <b>S4 Table.</b> Dominant phytoplankton mean abundance (±range) identified using Microscopy in each treatment (in cell mL<sup>-1</sup>). <b>S5 Table</b>. Statistical results for Pico- and Nano-phytoplankton (Cyano, Pico and Nano) concentration differences between treatments on two factors (Temperature x Zooplankton community [Zoo. Comm]), for Pre-Bloom, Bloom and Post-Bloom periods. Bold values indicate significant tests (p-values < 0.05). <b>S6 Table.</b> Dominant zooplankton taxa mean abundance (±range) identified using stereomicroscope in each treatment (in ind L<sup>-1</sup>).</p> <p>(DOCX)</p>

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Figshare
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2024-10-04
DOI
https://doi.org/10.1371/journal.pone.0308505.s001
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Marine and coastal ecosystems
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Abstract

<p><b>S1 Fig.</b> Median Chl <i>a</i> concentrations in the different treatments during pre-bloom (A), bloom (B), and post-bloom periods (C). Blue indicates the control treatment (C), red, green, and yellow indicated the heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. Boxplots with different letters differ are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has no stars or letters. <b>S2 Fig.</b> Median nutrient concentrations in the mesocosms between the different treatments for pre-bloom, bloom, and post-bloom periods. Nutrients are NH<sub>4</sub><sup>+</sup> (A, B, and C), NO<sub>2</sub><sup>-</sup> (D, E, and F), NO<sub>3</sub><sup>-</sup> (G, H, and I), SiO<sub>2</sub> (J, K, and L), and PO<sub>4</sub><sup>3-</sup> (M, N and O). Blue, control treatment (C); red, green, and yellow, the heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). A lack of asterisks and letters indicates RM-ANOVAs was nonsignificant. <b>S3 Fig.</b> Median concentrations of taxonomic pigments in the different treatments during pre-bloom, bloom, and post-bloom periods: Chl <i>b</i> (A, B, and C), Prasinoxanthin (D, E, and F), Zeaxanthin (G, H, and I), Alloxanthin (J, K, and L), 19HF (M, N, and O), Fucoxanthin (P, Q, and R), and Peridinin (S, T, and U). Blue, control (C); red, green, and yellow, heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post-hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has no stars or letters. <b>S4 Fig.</b> Median abundance of phytoplanktonic groups in the different treatments during pre-bloom, bloom, and post-bloom periods: Cyano (A, B, and C), Picoφ (D, E, and F), and Nanoφ (G, H, and I). Blue, control (C); red, green, and yellow, heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has neither stars nor letters. <b>S5 Fig.</b> Median abundance of protozooplankton (A, B and C) and micrometazooplankton (D, E and F) in the different treatments during pre-bloom, bloom, and post-bloom periods. Blue, control (C); red, green, and yellow, heated (T), mesozooplankton exclusion (MicroZ), and heated and mesozooplankton exclusion (TMicroZ) treatments, respectively. Significance level of RM-ANOVAs: * = <i>p</i>-value < 0.05; ** = <i>p</i>-value < 0.01; *** = <i>p</i>-value < 0.001. The letters indicate significant differences between treatments based on post hoc pairwise tests. Boxplots that share the same letter are not significantly different. When the letters differ, they are significantly different (<i>p-</i>value < 0.05). Nonsignificant RM-ANOVAs has neither stars nor letters. <b>S6 Fig.</b> Redundancy Analysis (RDA) between phytoplankton composition (pigment and cytometry and environmental parameters. (A) Pre-bloom period, (B) Bloom period, and (C) Post-Bloom periods. Dots present sampling dates community composition. Blue arrows present the projections of environmental parameters. Red labels present projection of the pigments and cytometry groups. The proximity between environmental parameters or pigments and cytometry groups and sampling dates indicates characteristic associations. Ellipses represents 95% confidence distribution interval of community for each treatment (C, T, MicroZ and TMicroZ). Ellipses were not represented for Pre-bloom (A) because they were overlapping and communities showed no significant differences. <b>S1 Table</b>. Light penetration mean in the mesocosms and diffuse attenuation coefficient (Kd). <b>S2 Table</b>. Statistical results for nutrients (NH<sub>4</sub><sup>+</sup>, NO<sub>2</sub><sup>-</sup>, NO<sub>3</sub><sup>-</sup>, Si, PO<sub>4</sub><sup>3-</sup>) concentration differences between treatments on two factors (Temperature x Zooplankton community [Zoo. Comm]), for Pre-Bloom, Bloom and Post-Bloom periods. Bold values indicate significant tests (p-values < 0.05). <b>S3 Table</b>. Statistical results for Pigments (Chl <i>b</i>, Prasi, Zea, Allo, 19HF, Fuco, Peri) concentration differences between treatments on two factors (Temperature x Zooplankton community [Zoo. Comm]), for Pre-Bloom, Bloom and Post-Bloom periods. Bold values indicate significant tests (p-values < 0.05). <b>S4 Table.</b> Dominant phytoplankton mean abundance (±range) identified using Microscopy in each treatment (in cell mL<sup>-1</sup>). <b>S5 Table</b>. Statistical results for Pico- and Nano-phytoplankton (Cyano, Pico and Nano) concentration differences between treatments on two factors (Temperature x Zooplankton community [Zoo. Comm]), for Pre-Bloom, Bloom and Post-Bloom periods. Bold values indicate significant tests (p-values < 0.05). <b>S6 Table.</b> Dominant zooplankton taxa mean abundance (±range) identified using stereomicroscope in each treatment (in ind L<sup>-1</sup>).</p> <p>(DOCX)</p>

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