Low glucose treatment induces mitochondrial network fusion in differentiated mature 3T3-L1 adipocytes.

<p>(A) Representative western blot showing change in mitofusin expression in 3T3-L1 cells during adipogenic differentiation. (B) Measurement of lipid droplet width from oil red O staining of day +12 3T3-L1 adipocytes treated with scrambled, <i>Mfn1</i>-targeted, or <i>Mfn2</i>-targeted siRNA on alternate days from day -2 to day +12 of differentiation. Data from four biological replicates. (C) Representative confocal images of 3T3-L1s cells at day +12 of differentiation under high glucose (HG) and low glucose (LG) conditions. Mitochondria were labelled using an anti-TOMM20 antibody (purple) and neutral lipids were stained with LipidTox (blue). Scale bars: 10 μm. (D) Quantification of mitochondrial morphology, and different mitochondrial parameters including number, area, and length in 225 μm<sup>2</sup> region of interests from (A). Stars indicate p-values following pairwise comparisons between groups: * p<0.5, *** p < .001.</p> <p>(PDF)</p>