Validation of the silencing efficiency in RNAi and VIGS assays.

(A) Quantitative RT-PCR to determine the expression of <i>NbUCH05</i>, <i>NbUCH12</i>, and <i>NbUCH15</i> in the experiments shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012882#ppat.1012882.g003" target="_blank">Fig 3</a>. Samples were taken 4 or 8 dpi. Expression values are relative to the expression of the housekeeping gene <i>NbEF1a</i>. Values indicate mean ± SE (n = 3 biological replicates). (B) Quantitative RT-PCR to determine the expression (silencing efficiency) of <i>NbPtr1</i>. Samples were taken 8 dpi. Expression values are relative to the expression of the housekeeping gene <i>NbEF1a</i>. Values indicate mean ± SE (n = 3 biological replicates). Each experiment was repeat at least three times with similar results. (PDF)