Increased adipogenesis in <i>Mfn1</i><sup>-/-</sup> MEFs even in setting of <i>Pparg2</i> over-expression.

<p><i>Pparg2</i> was over-expressed in MEFs to enhance their adipogenic differentiation capacity and cells were assessed on day +4 and +8 of protocols. (A) Western blot illustrating the relative Pparg2 over-expression in cell lines used compared to non-transduced wild-type cells. (B) Quantification of Pparg2 expression from the Western blot in panel (A). (C) Oil Red O staining differentiated MEFs compared to undifferentiated wild-type MEFs. (D) Fluorometric quantification of AdipoRed neutral lipid dye at day +4 & day +8. Each data point represents a separate biological experiment. Data is expressed relative to undifferentiated wild-type for each biological replicate. (E) Western blot for markers of adipocyte differentiation at day +8 from MEFs over-expressing <i>Pparg2</i>. All p-values represent pairwise comparisons between knock-outs and wild-type using t-tests, adjusted for multiple testing (p.adj). Data is representative of at least 3 independent replicates.</p> <p>(PDF)</p>