HMGB1 plays a crucial role in the inhibition of viral replication mediated by FBP.

<p>(A and B) HLCZ01 cells were infected with HCV (MOI, 0.01) for 72 h and then treated with the indicated concentration of FBP for 12 h. Subsequently, the mRNA levels of HMGB1 were assessed by qPCR (A), and the protein levels of HMGB1 were analyzed by western blot (B). (C-E) HEK293T cells or A549 cells were treated with FBP for 12 h and infected with VSV (MOI, 0.1) (C) or with HSV-1 (MOI, 0.1) (E) for 10 h. Huh7.5 cells were infected with HCV (MOI, 0.01) for 72 h and treated with FBP for 12 h (D). The cells were then treated with CHX (100 ng/μL) for 6 h, followed by western blot analysis of HMGB1. Data are presented as the mean ± SEM. NS, not significant. In (A), statistical analyses were performed with one-way ANOVA.</p> <p>(DOCX)</p>