Intravitreal injection of dTcap488 (6 nmol) to OS of Cy0410 resulted in significant multi-day low frequency background signal, and few foci of autoactivation.

Longitudinal Spectralis FAM images of Cy0410 OD (<b>A-G</b>) and OS (<b>H-N</b>) were paired based on time of non-cleavable, non-quenched 6 nmol TAT peptide probe dTcap488 intravitreal injection. Punctate fluorescent signals detected at OD were from previous HEA procedure and consecutive TcapQ488 intravitreal injections (<b>A-G</b>) to OD. <b>I’</b>) one day post the probe injection when total sensitivity setting was at 61 (all other images were set at 100). Arrow pointed hyperfluorescent signal at ONH, suggesting dTcap488 was actively transported towards the brain via RGC axons. The dTcap488 haze stayed in the vitreous for approximately 15 days before being cleared out. At Post 15 days (M) and 18 days (N) post dTcap488 injection, there was few detectable fluorescent signals at GCL, and the ONH was fluorescently hypo labeled. Scale bar (2 mm). (PDF)