S2 Fig -

Full alignment of CYCLOPS from 8 nodulating species and 1 non-nodulator (<i>Trema tomentosa</i>) (A). For <i>Datisca glomerata</i>, regions spanning disorder predicted (purple) and coil (light green) domains are depicted by a horizontal top bar. Note the presence of a disorder predicted domain at the N-terminus of <i>Datisca glomerata</i> that is only conserved in <i>Ceanothus thyrsiflorus</i> and <i>Alnus glutinosa</i>. Maximum-likelihood phylogenetic reconstruction of CYCLOPS/IPD3 (B). Multiple protein sequence alignment was produced after searching the orthologous group ENOG410IGIK at UniProtKB. Model legume orthologs are highlighted in pink, <i>Ceanothus thyrsiflorus</i> in green (GenBank accession MN388817), and <i>Datisca glomerata</i> in blue. (PDF)