Displayed all the result of agarose gel electrophoresis using GeneRuler<sup>TM</sup> 1kb DNA ladder (Fermentas) which denoted as M.

<p>(A) the genomic DNA extracted from <i>Bacillus licheniformis</i> IBRL-CHS2. The Lambda DNA/<i>Hind</i>III marker (Promega) is denoted as LM. The Genomic DNA is indicated by a white arrow in Lane 1. An agarose gel percentage of 0.7% was utilized for this analysis. (B, C) results of gel electrophoresis showing PCR products amplified with BLF and BLR (B) and the purified LipA gene, identified by the arrow, which is 615 bp in size (C). The samples were run on a 1% agarose gel for electrophoresis. (D) results of colony PCR products from 5 chosen white clones. The LipA gene, measuring 615 bp, is indicated by the arrow. (E) results of agarose gel electrophoresis (1%) following the EcoRI digestion of pGEM-LipA. Lane 1 shows the undigested pGEM- LipA in various conformations, resulting in bands of different sizes. The linear and monomer pGEM- LipA band is highlighted and labeled. In lane 2, digested pGEM- LipA shows the successful cleavage of the LipA gene from the pGEM-T Easy plasmid.</p> <p>(PDF)</p>