hdWGCNA co-expression module network and gene set enrichment analysis (A-E) Hub gene networks for each Neu co-expression module.

The top 25 hub genes ranked by kME are visualized. Nodes represent genes, and edges represent co-expression links. (F) Gene ontology enrichment analyses of hub genes from co-expression module M2 M3 M4. The dot size represented the number of genes. The color scale represented the adjusted p value. (G) Heatmap showing log2 (foldchange) in gene expression of the representative top300 apoptosis-related DEGs between control and cKO. The asterisks mean padjust < 0.05 in corresponding cells. (H) Percentages of annexin V⁺ and SYTOX^- cells in bone marrow neutrophil progenitor cells of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; MB, myeloblasts, MB; PM, promyelocytes, PM; MC, myelocytes; BM, bone marrow (n=10; mean ± SD). Mann-Whitney U test. (I) Representative FACS analysis of HL-60 cells treated with 0-20 µM MAP4K4 inhibitor (PF-06260933, PF) for staining of annexin V and SYTOX was shown (left). percentages of annexin V⁺ and SYTOX^- cells (right) (n=4; mean ± SD). Mann-Whitney U test. (J) Quantification of HL-60 cell numbers following treatment with a 10 µM concentration of the MAP4K4 inhibitor (PF-06260933, PF) was conducted over a period extending from 0 to 108 hours (n=4; mean ± SD). Mann-Whitney U test. (PDF)