Comparison of acquisition photobleaching in confocal and SIM modes.

A. Time lapse series of live HeLa cells labelled with SYBR Gold. SIM settings are described in Materials and Methods section. Briefly, frame time 1.8 s, 488 nm laser, 1% AOTF (corresponding to 13.5 microW, 0.54 mW/mm²). Confocal time series were acquired for the same field of view (50 by 50 μm), the same pixel size (50 nm) and same frame time (1.8 s) as for SIM images. Confocal imaging was performed under two settings: 1) blue, the same illumination power as for SIM (13.5 microW); 2) orange, illumination power reduced to 1.6 microW. B. Intensity profiles across nucleoids on confocal images acquired with 13.5 microW (blue) and 1.6 microW (orange). (PDF)