S9 Fig -

<p><b>(A)</b> Growth curves of ΔGiMlf and GiMlf-OE cell lines and their respective controls (n = 4). To establish whether the curves statistically differ, the CGGC permutation test (1000 permutations) was used. The growth of ΔGiMlf line was significantly impeded (P-value: 0.024), while there was no significant change in the growth of the GiMlf-OE line (P-value: 0.526) when compared to their respective control cell lines. <b>(B)</b> Comparison of the cell morphology of the ΔGiMlf and control cell line using expansion microscopy. Cells were stained with NHS ester, an anti-acetylated tubulin antibody, and an anti-PDI2 antibody. Scale bars: 10 μm. <b>(C)</b> Levels of the V5-tagged proteins in the control and ΔGiMlf cells in steady state (control) and after heat shock and 4h recovery. The proteins were detected by an anti-V5 antibody. TrxR was used as loading control. <b>(D)</b> Comparison of encysting cells (5 h and 48 h post induction) of ΔGiMlf and control cell lines using confocal fluorescence microscopy. The cells were stained with anti-CWP1 antibody (green). Nucleic DNA was stained with DAPI (blue). DIC images of corresponding cells are shown in corner of the merged images. Scale bars: 2 μm. <b>(E)</b> Schematic representation of <i>Giardia</i> energy metabolism with indicated downregulated enzymes (red). GK–glucokinase, GPI–glucose-6-phosphate isomerase, PFK–phosphofructokinase, FBA–fructose-bisphosphate aldolase, GA-3-P–glyceraldehyde-3-phosphate, TPI–triosephosphate isomerase, GAPDH–gleceraldehyd-3-phospate dehydrogenase, PGK–phosphoglycerate kinase, PGM—2,3-bisphosphoglycerate-independent phosphoglycerate mutase, ENOL–enolase, PEP–phosphoenolpyruvate, PK–pyruvate kinase, PEPCK–phosphoenolpyruvate carboxykinase (hypothetical protein GL50803_101278, homology inferred from HHpred prediction), PFOR–pyruvate-flavodoxin oxidoreductase, Fdox/red–oxidised/reduced ferredoxin, ADH–alcohol dehydrogenase, AAT–alanine aminotransferase, GluDH–glutamate dehydrogenase, ACS—Acetyl-CoA synthetase, MAL–malic enzyme, MDH–malate dehydrogenase, ATA–aspartate transaminase, GluS–Glutamate synthase, ASNase–L-asparaginase, GPDH–glycerol-3-phosphate dehydrogenase, IPS–inositol-3-phosphate synthase.</p> <p>(PDF)</p>