Optimization of assays to evaluate the humoral immune response against DENV.

<p>(A) flow cytometry-based neutralization assay measuring the percentage of infection. Vero CCL were in vitro infected with DENV-2 (red dot line) and ZIKV (black line) in presence or absence of serially diluted IFNAR-/- DENV2 challenged and ZIKV infected sera (B) Antibody-dependent enhancement assay. U937 cells were in vitro infected with DENV-2 (red dot line) and ZIKV (black line) in presence or absence of serially diluted IFNAR-/- DENV2 challenged and ZIKV infected sera. (C) Antibody-dependent cellular cytotoxicity assay. Jurkat reporter cells (effector cells) were incubated with Raji cell (target cells) in presence or absence of rituximab (anti-CD20) mAb. The data is shown as luminescence (RLU).</p> <p>(PDF)</p>