Levamisole sensitivity of <i>fshr-1</i> mutants is rescued by re-expression of <i>fshr-1</i> in known sites of <i>fshr-1</i> expression but is exacerbated by re-expression in non-endogenous muscle expression sites.

<p>(A-C, E) Box and whisker plots showing results of levamisole paralysis assays performed on wild type and <i>fshr-1(ok778)</i> mutant animals, as well <i>fshr-1</i> mutants re-expressing <i>fshr-1</i> (Rescue) in the indicated tissues (A, intestinal <i>Pges-1</i>, <i>ibtEx35</i>; B, glial <i>Pmir-228 ibtEx51</i>; C, neuronal <i>Prab-3 ibtEx34</i>; E, muscle <i>Pmyo-3</i>, <i>kjrEx39</i>). Worms were exposed on plates containing 200μM levamisole for 100 minutes and paralysis was assessed by nose tap. n = 9 plates of approximately 20 young adult animals per plate per strain were tested. (D) Box and whisker plots of swimming experiment data repeated at least twice with <i>fshr-1(ok778)</i> mutants with muscle-specific <i>fshr-1</i> re-expression. Note that muscle rescue, unlike intestinal, glial, or neuronal rescue, caused increased body bending rates that did not coincide with any restoration of wild type levamisole sensitivity, as seen with the other rescuing transgenes. One-way ANOVA and Tukey’s post hoc tests were used to compare the means of the datasets (*<i>p</i> ≤ 0.05, ** <i>p</i> ≤ 0.01, ***<i>p</i> ≤ 0.001; n.s., not significant).</p> <p>(PDF)</p>