VANGL2 and MARCH8 inhibit the activation of NLRP3 inflammasome in THP-1 cells.

(A) THP-1 cells were transfected with scramble siRNA or <i>VANGL2</i> siRNA for 24 h, followed by LPS (100 ng/ml) for 6 h and ATP (5 mM) for 30 min. Culture supernatants were collected to measure IL-1β release by ELISA. (B) THP-1 cells were transfected with scramble siRNA or <i>MARCH8</i> siRNA for 24 h, followed by LPS (100 ng/ml) for 6 h and ATP (5 mM) for 30 min. Culture supernatants were collected to measure IL-1β release by ELISA. ***<i>P</i> < 0.001, NS means not significant. (PDF)