Related to Fig 3.

<p>(A) The GSPC DAF-16::GFP nuclear-to-cytoplasmic ratio (N:C) in control animals (no TIR1), <i>sun-1p</i>::<i>TIR1</i> animals with and without auxin, and <i>eft-3p</i>::<i>TIR1</i> animals without auxin. The DAF-16::GFP N:C is elevated in s<i>un-1p</i>::<i>TIR1</i> and <i>eft-3p</i>::<i>TIR1</i> GSPCs without auxin treatment, compared to control, but increases further in <i>sun-1p</i>::<i>TIR1</i> animals after auxin treatment. Small dots represent individual GSPCs, larger dots represent the mean value per gonad arm/PZ. Boxplots show the median, interquartile range and most extreme values not considered statistical outliers. (B) Single z-slice sections through the middle of the germ line (orange outline; left), or a basal region through intestinal nuclei (blue arrow heads; right) in the same animals, showing DAF-16::GFP::AID expression in <i>daf-2</i>(RNAi) treated animals. As with DAF-2::AID::mNG, <i>eft-3p</i>::<i>TIR1</i> leads to partial somatic depletion of DAF-16::GFP::AID without the addition of auxin (compare top intestinal nuclei to those in either of the bottom 2 images). Auxin treatment leads to nearly complete DAF-16::GFP::AID depletion, in both germ line and soma, in <i>eft-3p</i>::<i>TIR1</i> animals, and robust germ line depletion in <i>sun-1p</i>::<i>TIR1</i> animals. Scale bar = 20 μm. Quantification of germ line DAF-16::GFP::AID fluorescence for a subset of the animals assayed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011351#pgen.1011351.g003" target="_blank">Fig 3E</a> is shown on the right. Dots represent the mean value per each gonad arm and one gonad arm was assessed per animal. Bar plots show the mean with error bars showing the standard deviation. For all plots, n.s. = <i>p</i> > 0.05, * = <i>p</i> < 0.05, ** = <i>p</i> <0.01, *** = <i>p</i> < 0.001. Summary statistics and statistical tests used for all figure panels are given in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011351#pgen.1011351.s010" target="_blank">S2 Data</a>.</p> <p>(PDF)</p>