Biological validation of TAT protein levels in total extract of metacyclics differentiated in the presence of TAU Pro-BCAAs.

<p><b>A.</b> Representative image of western blot for tyrosine aminotransferase (TAT) (~47 KDa) with total protein loading control stained with trichloroethanol (TCE) (left) and relative expression of TAT in the total extract of two biological replicates of metacyclics parasites (right). <b>B.</b> Average LFQ values for the four annotated TATs in metacyclics differentated in TAU Pro and TAU Pro-BCAAs. Graphs show average and standard deviation of three biological replicates. Statistically analysis using TAU Pro as the control was performed applying one-way ANOVA with multiple comparisons test (a = 0.05, ** p ≤ 0.01, *** p ≤ 0.001).</p> <p>(PDF)</p>