Additional analyses of the K17-dependent skin response to TPA treatments in vivo.

<p><b>(A)</b> Sections from WT and <i>Krt17-/-</i> mouse skin treated with acetone or single TPA were immunostained for Ly6g, K14, and nuclei (DAPI). Scale bars: 50 μm. epi, epidermis; derm, dermis; hf, hair follicle. Dashed lines depict the dermo-epidermal interface. <b>(B)</b> TUNEL staining of WT and <i>Krt17-/-</i> mouse ear skin 6 h after either double TPA Tx (24 h apart) or TPA-UVB combination Tx (24 h apart). Scale bar: 50 μm<b>. (C)</b> Percentage of TUNEL positive cells in epidermis after double TPA (data from B). <i>n</i> = 3 mice. Data are shown as mean ± SEM. Unpaired <i>t</i> test. <b>(D, E)</b> Mouse ear tissues were harvested 6 h after double acetone or dual TPA Tx, 24 h apart, and processed for bulk RNAseq analysis. Volcano plots reporting on changes in mRNA transcripts levels in TPA-Tx vs. vehicle-Tx for (D) WT skin and (E) <i>Krt17-/-</i> skin. Genes that are significantly up- or down-regulated by 8-fold or more (adj. <i>P</i> < 0.01) are highlighted in blue (WT) or red (<i>Krt17-/-</i>). <b>(F)</b> Comparison of genes significantly down-regulated after dual TPA-Tx vs. dual acetone-Tx in WT and <i>Krt17-/-</i> mouse skin (Cutoffs: FDR-adjusted <i>P</i> < 0.01, fold change > 8). <b>(G)</b> Panther overrepresentation test using Reactome pathways (FDR-adjusted <i>P</i> < 0.05) for the 268 genes showing significant up-regulated expression in both WT and <i>Krt17-/-</i> skin after double TPA Tx (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002779#pbio.3002779.g002" target="_blank">Fig 2I</a>). The source data used to derive the numerical values reported here can be found in <b><a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002779#pbio.3002779.s016" target="_blank">S1 Data</a></b>.</p> <p>(PDF)</p>