Assessment of low and high pigmentation melanocyte states (related to Fig 2).

<p>(A) Bright field images of day 7 B16 low and high pigmenting colonies. The bottom panel represents images of single cells sorted using imaging flow cytometry from the differentially pigmented day 7 sample. Scale bars are indicated. (B) Pearson’s correlation analysis between the average melanin content per cell estimated using NaOH method and the different parameters of imaging flow cytometer. (C) Linear regression analysis of mean brightfield intensity of imaging flow cytometer and average melanin content per cell estimated using NaOH method. (D) Distribution of pigmentation in B16 cells at day 7 of the progressive pigmentation model. (E) Side scatter intensity distribution (representing pigmentation) of B16 cells at days 0 and 7 of the progressive pigmentation model. Rectangle gate represents high pigment cells. All numerical data are listed in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002776#pbio.3002776.s011" target="_blank">S1 Data</a>.</p> <p>(DOCX)</p>