Gel filtration chromatograms of <i>S</i>. <i>cerevisiae</i> Upf1-HD (A) or Nmd4 (B) proteins used in our in vitro assays.

<p>(A) Size exclusion chromatography (S200 16/60) profiles obtained for pure Upf1-HD WT and mutant proteins, showing very similar retention times for these different proteins. The Coomassie stained SDS-PAGE analysis of the proteins present in the main peak is shown for every protein (WT and mutants) to show the purity of the final proteins. MW: Molecular weight ladder. (B) Size exclusion chromatography (S75 16/60) profiles obtained for pure His<sub>6</sub>-ZZ-Nmd4 WT and mutant proteins, showing very similar retention times for these different proteins. The Coomassie stained SDS-PAGE analysis of the proteins present in the main peak is shown for every protein (WT and mutants) to show the purity of the final proteins. MW: Molecular weight ladder. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002821#pbio.3002821.s024" target="_blank">S7 Data</a>.</p> <p>(PDF)</p>