pH and magnesium showed no or modest interactions with TbAd biosynthesis.

<p>(A) Heatmap of transcripts with <i>p</i> value < 0.01 in a contrast of pH 5.5 versus 6.6 in both the Mtb H37Rv and Δ<i>Rv3378c</i> strains measured by RNAseq. (B) Mass spectra of 1-TbAd, <i>m/z</i> 540.354, or <i>N</i><sup><i>6</i></sup>-TbAd (C), <i>m/z</i> 540.354, from replicate cultures grown in media containing 0.6, 6.0-, or 60-mM magnesium chloride (<i>n</i> = 3 replicates) with observed masses shown. The color key for magnesium concentration was shared for (B–E). Both combined and dithered peaks (inset) are shown to allow visualization of peak correspondence and individual samples. (D) Quantification and statistical analysis of 1-TbAd or <i>N</i><sup><i>6</i></sup>-TbAd (E) after lipidomics analysis using <i>limms</i> (Benjamini–Hochberg adjusted <i>p</i> value after Welch ANOVA). Raw data for S9 Fig ADE is provided in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.s013" target="_blank">S1 Data</a>. Raw RNAseq data is available in BioProject accession PRJNA1146031.</p> <p>(PDF)</p>