The <i>unc-31</i> mutation exacerbates the evoked but not the spontaneous release of inhibitory synaptic transmission in <i>cpx-1</i> mutants.

(<b>A</b>) Representative traces of eIPSCs recorded from the indicated strains. The lower traces of <i>cpx-1(ok1552)</i> (green) and <i>cpx-1(ok1552); unc-31(e928)</i> (blue) show a zoomed-in region from the upper traces. (<b>B</b>, <b>C</b>) Quantification of the eIPSC amplitude (<b>B</b>) and charge transfer (<b>C</b>) at each strain. <i>n</i> ≥ 8 animals. One-way ANOVA was performed (F_{(4, 48)} = 33.35, <i>P</i> < 0.0001 for <b>B</b>; F_{(4, 48)} = 21.53, <i>P</i> < 0.0001 for <b>C</b>). (<b>D</b>) Representative traces of mIPSCs recorded from the indicated strains at different timescales. (<b>E</b>, <b>F</b>) Quantification of the mIPSC frequency (<b>E</b>) and amplitude (<b>F</b>) at each strain. <i>n</i> ≥ 11 animals. One-way ANOVA was performed (F_{(4, 58)} = 1.786, <i>P</i> = 0.1439 for <b>E</b>; F_{(4, 58)} = 2.579, <i>P</i> = 0.0467 for <b>F</b>). The data are presented as box-and-whisker plots, with the median (central line), 25th–75th percentile (bounds of the box), and 5th–95th percentile (whiskers) indicated. Student’s <i>t</i> test was performed for comparisons of 2 groups, ## <i>P</i> < 0.01; ### <i>P</i> < 0.001. One-way ANOVA was used for comparisons of multiple groups, followed by Tukey’s range test, * <i>P</i> < 0.05; ** <i>P</i> < 0.01; *** <i>P</i> < 0.001; ns, not significant. The error bars represent the SEM. <i>N</i> = 3 independent replicates. All the raw data associated with this figure are available in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003023#pbio.3003023.s016" target="_blank">S1 Data</a>. (PDF)