Evoked excitatory neurotransmission regulation by UNC-31 is independent of peptidergic signaling.

(<b>A</b>) Representative mPSC traces recorded from WT, <i>kpc-1(tm1104)</i>, <i>aex-5(sa23)</i>, and <i>bli-4(e937)</i> worms. The right panels show a 0.5 s scale bar for clarity. (<b>B</b>, <b>C</b>) Quantification of the mPSC frequency (<b>B</b>) and amplitude (<b>C</b>) across the indicated genotypes, as shown in panel (<b>A</b>). <i>n</i> ≥ 10 animals. One-way ANOVA was performed (F_{(3, 38)} = 0.1245, <i>P</i> = 0.945 for <b>B</b>; F_{(3, 38)} = 1.652, <i>P</i> = 0.1936 for <b>C</b>). (<b>D</b>–<b>F</b>) Representative traces and quantification of evoked EPSCs recorded from the mentioned genotypes. <i>n</i> ≥ 10 animals. One-way ANOVA was performed (F_{(3, 47)} = 0.4829, <i>P</i> = 0.6958 for <b>E</b>; F_{(3, 47)} = 1.201, <i>P</i> = 0.3197 for <b>F</b>). The data are presented as box-and-whisker plots, with the median (central line), 25th–75th percentile (bounds of the box), and 5th–95th percentile (whiskers) indicated. One-way ANOVA was performed; ns, not significant. The error bars represent the SEM. <i>N</i> = 3 independent replicates. All the raw data associated with this figure are available in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003023#pbio.3003023.s016" target="_blank">S1 Data</a>. (PDF)