Flow cytometry gating strategy to assess cell type markers in differentiating neural stem and progenitor cells.

<p>(A,B) Flow cytometry scatter plots of representative <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> (A) and <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> (B) neural stem and progenitor cells (NSPCs) grown in differentiation medium (without FGF2 or EGF and with 1% FCS) for 5 days. Side scatter (SSC) and forward scatter (FSC) width (W) and height (H) were used to exclude cell doublets; SSC and FSC areas (A) were used to exclude cell debris; a fixable live/cell death marker was used to select live cells; fluorescently labelled antibodies (see methods) were used to detect astrocytes [glial fibrillary acidic protein positive cells (GFAP<sup>+</sup>) and S100 protein, beta polypeptide, neural positive (S100ß<sup>+</sup>)], oligodendrocytes (monoclonal antibody O4<sup>+</sup>) and neurons (ßIII-tubulin<sup>+</sup>).</p> <p>(PDF)</p>