FLEXI RNAs from the same host gene can differ widely in expression levels.

<p>(A) IGV screenshots showing examples of differences in abundance of FLEXI RNAs transcribed from the same host gene reflecting post-transcriptional differences in splicing efficiency, alternative splicing, or differential stability of FLEXI RNAs. Gene maps for different RNA isoforms generated by alternative splicing of FLEXI RNAs are shown at the top and bottom with introns from the 5’ to 3’ RNA end named A to F. Reads mapping to exons or non-FLEXI introns were omitted for clarity. Reads for FLEXIs from different cellular RNA samples are color coded as shown in the Figure. NTA, non-templated nucleotides added to the 3’ end of cDNAs during TGIRT-seq library preparation. Two FLEXIs in the top panel had indels at short runs of 3’ U residues, likely reflecting a previously reported tendency for TGIRT enzyme slippage at such locations [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011416#pgen.1011416.ref086" target="_blank">86</a>]. (B) IGV screen shots showing differences in expression levels for long genes with labels omitted for better visualization of multiple FLEXIs from the same host gene.</p> <p>(PDF)</p>