Replicate agreement and sample diversity as quality metrics.

(A) Correlation of library spacer counts per million (CPM) across the two biological replicates for samples containing polymyxin B at concentrations of 0.5 ug/mL (left) or 1 ug/mL (right). Count density represented by contour map; right graph shows over-depletion of guides. (B) Population diversity (N_b) for polymyxin B replicates. Samples with nontargeting guide complexity below the cutoff (dotted line) were excluded from analyses. (C) Heatmap showing correlations of spacer CPMs between solvent controls. All solvent control samples show r≥0.97. (PDF)