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<p>Table A. Comparison results of scMSI and MSIsensor for different proportions of primary clones. Table B. Performance comparison of scMSI and MSIsensor in different sequencing depths. Table C. Comparison results of scMSI and MSIsensor for different clone numbers. Table D. When the number of subclones is 2 the detection results under different density distributions of microsatellite lengths in subclones at all levels. Table E. When the number of subclones is 3, the detection results under different density distributions of microsatellite lengths in subclones at all levels. Table F. When the number of subclones is 4, the detection results under different density distributions of microsatellite lengths in subclones at all levels. Table G. Cases with clonal MMR deficiencies. Table H. 15 microsatellite sites for detection. Table I. Classification of clonal microsatellite status for case 3. Table G. Classification of clonal microsatellite status for case 4. Table K. Classification of clonal microsatellite status for case 5. Table L. Classification of clonal microsatellite status for case 6. Table M. Classification of clonal microsatellite status for case 7. Table N. Classification of clonal microsatellite status for case 8. Table O. Classification of clonal microsatellite status for case 9. Table P. Classification of clonal microsatellite status for case 10. Table Q. Classification of clonal microsatellite status for case 11. Table R. Classification of clonal microsatellite status for case 12. Table S. Classification of clonal microsatellite status for case 13. Table T. Classification of clonal microsatellite status for case 14. Table U. Classification of clonal microsatellite status for case 15. Table V. Classification of clonal microsatellite status for case 16. <b>Fig A.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 3. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 expression in approximately half of the tumor cells. <b>Fig B.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 4. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 expression in approximately half of the tumor cells. <b>Fig C.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 5. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in focal tumor cells. <b>Fig D.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 6. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in the majority of tumor cells. <b>Fig E.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 7. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in approximately half of the tumor cells. <b>Fig F.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 8. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in approximately half of the tumor cells. <b>Fig G.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 9. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MSH2 and MSH6 protein in the majority of tumor cells. <b>Fig H.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 10. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in the majority of tumor cells. <b>Fig I.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 11. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in the majority of tumor cells. <b>Fig G.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 12. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in focal tumor cells. <b>Fig K.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 13. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MLH1 and PMS2 protein in the majority of tumor cells. <b>Fig L.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 14. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MSH2 and MSH6 protein in the majority of tumor cells. <b>Fig M.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 15. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MSH2 and MSH6 protein in focal tumor cells. <b>Fig N.</b> IHC detection map and PCR microsatellite detection site length distribution map of case 16. (A) BAT26 microsatellite length distribution map (B) NR24 microsatellite length distribution map (C) BAT25 microsatellite length distribution map (D) NR27 microsatellite length distribution map (E) NR21 microsatellite length distribution map. (F) The figures of clonal immunohistochemical loss of MMR expression in tumor cells: clonal loss of MSH2 and MSH6 protein in focal tumor cells.</p> <p>(DOCX)</p>