Analysis of RNAs in nuclear and cytoplasmic fractions from cultured cells.

<p>Scatter plots comparing enrichment of all and subtypes of cellular RNAs in cytoplasmic and nuclear fractions from HeLa S3, K-562, MDA-MB-231, and MCF7 cells. RNA subtypes are color coded as shown to the right of the scatter plots. Mitochondrial RNAs, tRNAs, Vault RNAs, Y RNA, and 7SL RNA were enriched in the cytoplasmic RNA fraction, while 7SK RNA and snRNAs were enriched in the nuclear RNA fraction. Most snoRNAs (357 to 457 in different cell types) had higher normalized counts in nuclear fractions than in cytoplasmic fractions with only 20–49 snoRNAs having higher normalized counts in cytoplasmic fractions.</p> <p>(PDF)</p>